Origin of replication – The origin of replication is essential for the replication of plasmid vectors inside bacterial host cells. Fluorescent markers enable researchers to select for positively transfected/transduced cells by visualization under a fluorescence microscope or by fluorescence activated cell sorting (FACs). Cells not carrying the vector on the other hand are non-resistant to the antibiotic and are therefore, killed in its presence. Drug-selection markers function by conferring resistance against the respective antibiotic in cells carrying the vector with the marker. Two types of markers are commonly used for selection of positively transfected/transduced mammalian cells – drug-selection markers and fluorescent protein markers. Selection marker – A selection marker enables the experimenter to successfully identify cells that have been positively transfected or transduced with the vector. Additionally, polyadenylation is essential for the nuclear export of the mRNA into the cytosol and the translation of the protein by ribosomes. Incorporating the polyA signal in a vector results in polyadenylation of the mRNA which protects the mRNA from being degraded by nucleases and phosphatases. PolyA signal – polyA stands for polyadenylation signal sequence and is responsible for the transcription termination and polyadenylation of the transcript produced from the upstream ORF sequence. When multiple proteins are needed to be expressed simultaneously, ORFs corresponding to those proteins can be placed under the same promoter separated by linkers to be expressed as a polycistron. The ORF is vital for expression vectors and is placed downstream of the promoter. ORF – ORF stands for the open reading frame of the user’s GOI which is ultimately translated into functional protein. Additionally, promoters can be of variable strengths (weak, medium or strong) based on the level of target gene expression that can be achieved by them. Promoters can be either ubiquitous, driving gene expression in all cell types, or they can be tissue-specific, driving gene expression only in specific cell types. The selection of the promoter for a vector depends on the desired location and level of target gene expression. Promoter – The promoter is a critical component of all expression vectors since it drives transcription of the GOI lying downstream of it. Species – We offer vectors for transgene expression in a variety of species including mammals, zebrafish, Drosophila, plants, bacteria, yeast and insects. CRISPR-based gene editing and regulation.piggyBac, Tol2 and sleeping beauty): for transposon-mediated gene transferĪpplication – Our rich vector collection includes vectors for a variety of applications, such as: lentivirus, AAV, adenovirus and retrovirus): for viral transduction-based gene delivery Regular plasmid: for conventional transfection-based gene delivery.Vector backbone – We offer a wide range of vector backbones, including You can start designing your own vectors by clicking on Design My Vectors from our homepage, which will allow you to select the appropriate vector system suitable for your experiment from close to 200 available systems:
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Free bioinformatic tools to assist you with vector design and analysisĭesigning your custom vectors from Vectorbuilder’s user-friendly online vector design platform is extremely easy and offers you with several advantages as described below: Available vector systems.Rich and comprehensive educational guides on vector systems and vector components.Detailed vector maps with rich annotation of vector components.Extensive databases of vector components including promoters, ORFs, markers, epitope tags, shRNAs and more.Diverse collection of vector backbones including regular plasmid, lentivirus, AAV, adenovirus, MMLV, piggyBac and more.In addition to designing your vectors, you can order custom cloning of your designed vectors along with related services such as virus packaging and plasmid DNA preparation from our platform. Whether you have a VectorBuilder account or not, you can have free and unlimited access to our online vector design platform which allows you to design various custom vectors with just a few mouse clicks. VectorBuilder offers a highly innovative web-based platform that combines sophisticated yet intuitive vector designing with streamlined online ordering.
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